Quantitative Proteomic Approach Reveals Altered Metabolic Pathways in Response to the Inhibition of Lysine Deacetylases in A549 Cells under Normoxia and Hypoxia

Growing evidence is showing that acetylation plays an essential role in cancer, but studies on the impact of KDAC inhibition (KDACi) on the metabolic profile are still in their infancy. Here, we analyzed, by using an iTRAQ-based quantitative proteomics approach, the changes in the proteome of KRAS-mutated non-small cell lung cancer (NSCLC) A549 cells in response to trichostatin-A (TSA) and nicotinamide (NAM) under normoxia and hypoxia. Part of this response was further validated by molecular and biochemical analyses and correlated with the proliferation rates, apoptotic cell death, and activation of ROS scavenging mechanisms in opposition to the ROS production. Despite the differences among the KDAC inhibitors, up-regulation of glycolysis, TCA cycle, oxidative phosphorylation and fatty acid synthesis emerged as a common metabolic response underlying KDACi. We also observed that some of the KDACi effects at metabolic levels are enhanced under hypoxia. Furthermore, we used a drug repositioning machine learning approach to list candidate metabolic therapeutic agents for KRAS mutated NSCLC. Together, these results allow us to better understand the metabolic regulations underlying KDACi in NSCLC, taking into account the microenvironment of tumors related to hypoxia, and bring new insights for the future rational design of new therapies.     

零析水水泥浆配合双凝双压固井工艺在泽74-1X井固井作业中的应用

泽74－1X井是为评价泽74断块奥陶系潜山油气情况，在泽74－1井开窗侧钻的一口评价井，该井井斜变化大，井眼轨迹呈螺旋型，地层特性复杂。该井通过采用ZJ－2低失水、零析水水泥浆体系配合双凝双压固井工艺，固井施工顺利，全井主要封固段固井质量良好。现场应用表明，ZJ－2降失水水泥浆体系具有流动性好、水泥浆初始稠度低、析水为零等特点，稠化时间易于控制，十分适合大斜度井和井眼轨迹不佳的井的固井要求，能够很好地保护油气层；双凝双密度水泥浆体系应用于压力变化大的超长裸眼封固段固井，施工安全，固井质量好，只要水泥浆密度设计合理，工程上采取小排量替浆，可防止固井漏失，提高作业成功率。     

钻井液用BDY-1便携式堵漏仪

在堵漏过程中，对堵漏材料和堵漏浆配方的优选是决定堵漏成功的关键。为了能在现场对堵漏进行评价，研制出BDY－1便携式堵漏仪，该堵漏仪主要由不锈钢钢柱圈钢，压力表等加工而成，该堵漏仪体积小，重量轻，价格低，仅为国产堵漏仪的1／20。试验结果表明BDY－1便携式堵漏仪操作简便，可快速对堵漏材料进行评价并筛选出堵漏配方；数据重复性好，准确可靠，能达到进口或国产堵漏仪的水平；在模拟漏层选定后，可采用多套压力梯度，对堵漏浆封堵效果进行评价，在压力梯度选定后，可评价堵漏浆对不同类型漏层的封堵效果；如果扩大压力范围，可对封堵后的漏层进行破裂试验，从而确定合理的钻井施工参数；适用现场操作和应用。     

沥青延度与流变特性关系研究分析(一)

通过对两种基质沥青进行不同温度及不同速率下的延度试验，找出它们的流变特性，并从微观上进行分析，最后与路用性能相联系，又通过TFOT试验找出流变特性对耐老化性之间的关系，最后得出，沥青的流变特性可通过延度试验来表征，它能够较好地反映沥青的路用性能。     

矿渣浆降失水剂GJ-1的应用

针对矿渣浆水灰比较大、固相含量相对较少、自由水比较多、失水量较大的特点，用实验的方法优选出了降失水剂GJ－1。实验和现场应用表明，GJ－1具有许多优点：（1）用量少，降失水效果显著；（2）配伍性良好，GJ－1的加入不影响激活剂、助剂、分散剂以及其它添加剂的作用效果；（3）耐温性良好，耐碱性比较好；（4）具有润湿、润滑作用，有利于泵送；（5）有一定的缓凝作用；（6）提高矿渣石的高温高压强度，GJ－1加量在0．6％以上时能将矿渣石的高温高压强度提高3－4MPa；（7）增粘不明显；（8）具有一定的悬浮能力；（9）强化矿渣浆的直角稠化，加入GJ－1的矿渣浆可以应用于防窝固井技术，能够解决长封固段井的固井难题和低密度矿渣浆的沉降稳定性问题，保证施工安全，提高固井质量。     

Engineering Geological Problems of Levee Along Middle and Lower Reaches of Yangtze River

1　ＩｎｔｒｏｄｕｃｔｉｏｎＢｅｃａｕｓｅｏｆｔｈｅｃｏｍｐｌｉｃａｔｅｄｓｕｂｓｏｉｌｐｒｏｆｉｌｅ ,ｔｈｅｌｅｖｅｅａｌｏｎｇｍｉｄｄｌｅａｎｄｌｏｗｅｒｒｅａｃｈｅｓｏｆＹａｎｇｔｚｅ ,ａｂｏｕｔ 36 0 0ｋｍｌｏｎｇ ,ｈａｓｍａｎｙｈｉｄｄｅｎｐｅｒｉｌｓ .Ａｍｏｎｇｔｈｅｄａｎｇｅｒｏｕｓｅｖｅｎｔｓｄｕｒｉｎｇ 1998’ｓｆｌｏｏｄｓｅａｓｏｎｉｎｔｈｅｗｈｏｌｅｖａｌｌｅｙ ,12 .7%ｏｃ ｃｕｒｒｅｄａｌｏｎｇｔｈｅｔｒｕｎｋｓｔｒｅａｍｄｙｋｅｓ,ｏｆｗｈｉｃｈ 6 98ｗｅｒｅｔｈｅｍｏｓｔｄ…     

深化水管体制改革促进水务事业持续发展

普洱市位于云南省西南部，面积4．5万km^2，与越南、老挝、缅甸三国接壤，国境线长486km。新中国成立以来，历届党委、政府高度重视普洱市水利事业的发展，把除水害、兴水利作为造福人民、荫及子孙的百年大计。到2006年年底止，全市累计建成蓄引工程49163件，其中：蓄水工程637件（中型水库10座、小型水库262座、塘坝365座），设计库容47556万m^3：引水工程48526件，年供水总能力11亿m^3。[第一段]     

Synthesis, characterization and devitrification behaviour of an yttrium containing boroaluminate glass

The glass forming region in the B₂O₃-Al₂O₃-Y₂O₃ composition diagram has been determined by a melting and quenching procedure at temperatures up to 1800°C. Different physical characteristics (density, coefficient of thermal expansion, glass transition and crystallization peak temperatures) have been determined for a 35B₂O₃-40Al₂O₃-25Y₂O₃ glass composition (in mol.%). By using a predictive model and some NMR structural data, different elastic moduli (Young's modulus, bulk modulus, shear modulus and Poisson's ratio) have been calculated. The devitrification behaviour has also been studied. Internal crystallization is the dominant mechanism and a new (Y, Al)BO₃ ternary phase has been characterized by X-ray powder diffraction. The temperature and time nucleation dependence have been determined from DTA experiments as well as the crystallization kinetics (i.e. the Avrami exponent and the activation energy for crystal growth).     

用不稳定试井资料研究油藏储集层流动系统模式：以准东油区为例

从不稳定试井Ｈｏｒｎｅｒ曲线形态分类入手，结合油藏动态特征对油藏储集层流动系统进行分类，并初步确定出各类流动系统的特征参数及其相应的开采特点。根据不稳定试井Ｈｏｒｎｅｒ曲线形态，可以把相应的储集层流动系统划分为三大类（孔隙流动系统、双重介质流动系统、裂缝流动系统）五个亚类。特别提出了双重介质孔缝串并流动系统组合模式。这无论对新区开发方案的编制，还是老区开发政策的制定都具有重要的借鉴参考价值。     

令牌多环网性能—可靠性综合评价

网络的性能与可靠性已分别得到广泛的研究,然而,怎样分析具有容错机制的环网的性能仍属有待开拓的研究领域,本文提出了一种分析具有自修复机制的多环网络系统的方法,并给出了报文传输时间分布的拉氏变换式,平均等待时间及平均队列长度公式,分析结果可用于评估故障与修复过程对实时令牌环网的实时响应特性的影响,对基于LAN的分布式实时系统设计具有指导意义。